3 Fold Dilution Formula:
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A 3-fold dilution means reducing the concentration of a solution to one-third of its original value by adding an appropriate amount of diluent. This is a common procedure in laboratory settings for preparing samples of desired concentrations.
The calculator uses the simple dilution formula:
Where:
Explanation: The formula divides the initial concentration by 3 to calculate the concentration after a 3-fold dilution.
Details: Accurate dilution calculations are essential in laboratory work, pharmaceutical preparations, and chemical analysis to achieve desired concentrations for experiments, treatments, or analytical procedures.
Tips: Enter the initial concentration in any consistent unit. The calculator will provide the final concentration after 3-fold dilution in the same units.
Q1: What does "3-fold dilution" mean?
A: A 3-fold dilution means the concentration is reduced to one-third of the original value by adding two parts diluent to one part original solution.
Q2: Can I use this for any concentration units?
A: Yes, the calculator works with any concentration units (mg/mL, M, %, etc.) as long as you maintain consistency between input and output.
Q3: How do I prepare a 3-fold dilution in practice?
A: Mix 1 part of the original solution with 2 parts of diluent to achieve a 3-fold dilution.
Q4: What if I need a different dilution factor?
A: This calculator is specifically for 3-fold dilutions. For other dilution factors, you would need to use the general formula C_f = C_i / dilution_factor.
Q5: Are there limitations to this calculation?
A: This assumes perfect mixing and no chemical interactions between the solute and diluent. The calculation may need adjustment for very concentrated solutions or specific chemical properties.