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Calculation of Dilution Factor in Analysis

Dilution Factor Formula:

\[ DF = \frac{C_i}{C_f} \]

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1. What is Dilution Factor?

The dilution factor (DF) is a dimensionless quantity used in analytical chemistry to express the ratio of the initial concentration to the final concentration of a solution after dilution. It indicates how many times the original solution has been diluted.

2. How Does the Calculator Work?

The calculator uses the dilution factor formula:

\[ DF = \frac{C_i}{C_f} \]

Where:

Explanation: The dilution factor represents the factor by which the original solution has been diluted. A DF of 10 means the solution has been diluted 10 times.

3. Importance of Dilution Factor Calculation

Details: Accurate dilution factor calculation is crucial for preparing solutions of desired concentrations, analytical measurements, and ensuring experimental reproducibility in chemical and biological laboratories.

4. Using the Calculator

Tips: Enter both initial and final concentrations in the same units. Values must be positive, and the final concentration should be less than or equal to the initial concentration.

5. Frequently Asked Questions (FAQ)

Q1: What units should I use for concentration?
A: You can use any concentration units (mg/mL, M, %, etc.) as long as both initial and final concentrations use the same units.

Q2: What does a dilution factor of 1 mean?
A: A DF of 1 means no dilution occurred - the initial and final concentrations are identical.

Q3: How is dilution factor related to dilution ratio?
A: Dilution factor is the inverse of the dilution ratio. For example, a 1:10 dilution has a dilution factor of 10.

Q4: Can dilution factor be less than 1?
A: No, dilution factor should always be ≥1 since final concentration after dilution cannot exceed initial concentration.

Q5: How do I prepare a solution with a specific dilution factor?
A: To achieve a specific DF, dilute 1 part of stock solution with (DF-1) parts of diluent.

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