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Dilution Factor Formula:
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The Dilution Factor (DF) is a dimensionless value used in hematology to calculate the appropriate dilution of red blood cells (RBCs) for accurate counting and analysis. It represents the ratio of the total volume (RBC volume plus diluent volume) to the original RBC volume.
The calculator uses the Dilution Factor formula:
Where:
Explanation: The formula calculates how many times the original RBC sample has been diluted by adding a specific volume of diluent.
Details: Accurate dilution factor calculation is crucial for obtaining correct RBC counts in hematological analyses. Proper dilution ensures that cell counts fall within the measurable range of automated hematology analyzers or manual counting methods.
Tips: Enter RBC volume and diluent volume in microliters (μL). Both values must be positive numbers, with RBC volume greater than zero.
Q1: Why is dilution necessary for RBC counting?
A: Dilution is necessary because undiluted blood contains too many RBCs for accurate counting. Proper dilution brings the cell concentration into a range that can be accurately measured by counting instruments.
Q2: What are typical dilution factors used in hematology?
A: Typical dilution factors range from 1:100 to 1:500 for automated analyzers, depending on the specific instrument and methodology used.
Q3: What type of diluent is used for RBC counting?
A: Isotonic solutions such as normal saline (0.9% NaCl) or specially formulated diluents that preserve cell integrity and prevent clotting are commonly used.
Q4: How does dilution factor affect the final RBC count?
A: The final RBC count is obtained by multiplying the counted number of cells by the dilution factor. An incorrect dilution factor will lead to inaccurate results.
Q5: Can this formula be used for other blood cell types?
A: Yes, the same dilution factor calculation applies to white blood cell (WBC) and platelet counting, though the optimal dilution ranges may differ for different cell types.