1. What is Dilution Factor?

The Dilution Factor (DF) is a dimensionless value that represents the ratio of the total volume after dilution to the original volume of the concentrated solution. It indicates how much a solution has been diluted.

2. How Does the Calculator Work?

The calculator uses the dilution factor formula:

Where:

• \( DF \) — Dilution Factor (dimensionless)
• \( V_c \) — Cell volume (mL)
• \( V_m \) — Media volume (mL)

Explanation: The formula calculates the factor by which the original cell suspension is diluted when media is added.

3. Importance of Dilution Factor Calculation

Details: Accurate dilution factor calculation is crucial for cell culture experiments, drug testing, and various biological assays where precise dilution ratios are required for consistent and reproducible results.

4. Using the Calculator

Tips: Enter cell volume and media volume in mL. Both values must be positive numbers, and cell volume must be greater than zero.

5. Frequently Asked Questions (FAQ)

Q1: What does a dilution factor of 1 mean?
A: A dilution factor of 1 means no dilution has occurred (V_m = 0).

Q2: How is dilution factor different from dilution ratio?
A: Dilution factor represents the total volume divided by the original volume, while dilution ratio expresses the ratio of original solution to diluent (e.g., 1:10).

Q3: Can dilution factor be less than 1?
A: No, dilution factor cannot be less than 1 as it represents the factor by which the original volume increases.

Q4: When should I use this calculation?
A: This calculation is essential when preparing cell cultures, performing serial dilutions, or any laboratory procedure requiring precise dilution measurements.

Q5: How does dilution factor affect concentration?
A: The final concentration equals the original concentration divided by the dilution factor.