Dilution Calculation From Stock Solution

Dilution Formula:

\[ V_{stock} = \frac{C_{final} \times V_{final}}{C_{stock}} \]

Stock Volume (V_stock):

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1. What is Dilution Calculation From Stock Solution?

Dilution calculation from stock solution is a fundamental laboratory technique used to determine the volume of a concentrated stock solution needed to prepare a solution of desired concentration and volume. This calculation is essential in chemistry, biology, and pharmaceutical preparations.

2. How Does the Calculator Work?

The calculator uses the dilution formula:

\[ V_{stock} = \frac{C_{final} \times V_{final}}{C_{stock}} \]

Where:

\( V_{stock} \) — Volume of stock solution needed (mL)
\( C_{final} \) — Final desired concentration (M)
\( V_{final} \) — Final desired volume (mL)
\( C_{stock} \) — Concentration of stock solution (M)

Explanation: The formula is derived from the principle of conservation of mass, where the amount of solute remains constant before and after dilution.

3. Importance of Dilution Calculations

Details: Accurate dilution calculations are crucial for preparing solutions with precise concentrations, ensuring experimental reproducibility, and maintaining safety standards in laboratory work.

4. Using the Calculator

Tips: Enter final concentration in M (molar), final volume in mL, and stock concentration in M. All values must be positive numbers. The calculator will determine the required volume of stock solution.

5. Frequently Asked Questions (FAQ)

Q1: What if my concentrations are in different units?
A: Ensure all concentration values are in the same units before calculation. Convert if necessary using appropriate conversion factors.

Q2: Can this formula be used for serial dilutions?
A: Yes, the same principle applies to serial dilutions, though each dilution step must be calculated separately.

Q3: What is the maximum accuracy of this calculation?
A: The calculation is mathematically exact. Practical accuracy depends on the precision of your measurements and equipment.

Q4: Does this work for non-aqueous solutions?
A: Yes, the dilution formula applies to any solution where concentration is expressed consistently, regardless of solvent.

Q5: How do I handle very small volumes?
A: For microliter volumes, use appropriate precision pipettes and consider the limitations of your measuring equipment.