1. What is the Dilution Formula?

The dilution formula calculates the final concentration of a solution after dilution. It is based on the principle of conservation of mass, where the amount of solute remains constant before and after dilution.

2. How Does the Calculator Work?

The calculator uses the dilution formula:

Where:

• \( C_f \) — Final concentration (ng/μL)
• \( C_i \) — Initial concentration (ng/μL)
• \( V_i \) — Initial volume (μL)
• \( V_f \) — Final volume (μL)

Explanation: The formula calculates the final concentration by multiplying the initial concentration by the ratio of initial to final volume.

3. Importance of Dilution Calculation

Details: Accurate dilution calculations are essential in laboratory settings for preparing solutions with specific concentrations, ensuring experimental consistency, and maintaining proper reagent concentrations.

4. Using the Calculator

Tips: Enter initial concentration in ng/μL, initial volume in μL, and final volume in μL. All values must be valid (greater than 0).

5. Frequently Asked Questions (FAQ)

Q1: What units should I use for this calculator?
A: This calculator uses ng/μL for concentration and μL for volume. Ensure all inputs are in these units for accurate results.

Q2: Can I use this for other concentration units?
A: The formula works for any consistent concentration units, but this calculator is specifically designed for ng/μL.

Q3: What if my final volume is smaller than initial volume?
A: This would represent concentration rather than dilution, but the formula still applies mathematically.

Q4: How precise are the calculations?
A: Results are calculated with high precision (4 decimal places) to accommodate laboratory accuracy requirements.

Q5: Can this calculator handle very small volumes?
A: Yes, the calculator accepts volumes as small as 0.0001 μL, suitable for micro-volume dilutions.