Dilution Calculator Tocris Bioscience

Tocris Dilution Formula:

\[ V_s = \frac{C_f \times V_f}{C_s} \]

Stock Volume (V_s):

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1. What is the Tocris Dilution Formula?

The Tocris dilution formula calculates the volume of stock solution needed to achieve a desired concentration in a final solution volume. This is essential for preparing accurate dilutions in laboratory research and experiments.

2. How Does the Calculator Work?

The calculator uses the Tocris dilution formula:

\[ V_s = \frac{C_f \times V_f}{C_s} \]

Where:

\( V_s \) — Stock volume (μL)
\( C_f \) — Final concentration (μM)
\( V_f \) — Final volume (mL)
\( C_s \) — Stock concentration (mM)

Explanation: The formula calculates the required volume of stock solution to dilute to achieve the desired final concentration in the specified final volume.

3. Importance of Accurate Dilution Calculations

Details: Precise dilution calculations are crucial for experimental reproducibility, accurate dosing in biological assays, and ensuring consistent results in research applications.

4. Using the Calculator

Tips: Enter final concentration in μM, final volume in mL, and stock concentration in mM. All values must be positive numbers greater than zero.

5. Frequently Asked Questions (FAQ)

Q1: Why are different concentration units used in the formula?
A: The formula uses μM for final concentration and mM for stock concentration to maintain consistency with common laboratory practices and concentration ranges.

Q2: What if my stock concentration is in a different unit?
A: Convert your stock concentration to mM before entering. For example, 1 M = 1000 mM, 1 μM = 0.001 mM.

Q3: Can this calculator be used for serial dilutions?
A: This calculator calculates single-step dilutions. For serial dilutions, multiple calculations would be needed for each dilution step.

Q4: What is the typical accuracy of this calculation?
A: The calculation is mathematically precise. Actual accuracy depends on the precision of your measurements and pipetting technique.

Q5: How should I handle very small volumes?
A: For volumes below 1 μL, consider using concentrated stock solutions or alternative dilution strategies to maintain pipetting accuracy.