Dilution Calculator Tocris

Tocris Dilution Formula:

\[ V_s = \frac{C_f \times V_f}{C_s} \]

Stock Volume (V_s):

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1. What is the Tocris Dilution Formula?

The Tocris dilution formula calculates the volume of stock solution needed to achieve a desired concentration in a final solution volume. It's commonly used in laboratory settings for preparing solutions with precise concentrations.

2. How Does the Calculator Work?

The calculator uses the Tocris dilution formula:

\[ V_s = \frac{C_f \times V_f}{C_s} \]

Where:

\( V_s \) — Stock volume (μL)
\( C_f \) — Final concentration (μM)
\( V_f \) — Final volume (mL)
\( C_s \) — Stock concentration (mM)

Explanation: The formula calculates the required volume of stock solution to dilute in order to achieve the desired final concentration in the specified final volume.

3. Importance of Dilution Calculations

Details: Accurate dilution calculations are essential for preparing experimental solutions with precise concentrations, ensuring reproducibility and reliability in scientific research and laboratory work.

4. Using the Calculator

Tips: Enter final concentration in μM, final volume in mL, and stock concentration in mM. All values must be positive numbers greater than zero.

5. Frequently Asked Questions (FAQ)

Q1: Why are different units used for concentration?
A: Stock concentrations are typically in mM while final concentrations are in μM to accommodate common laboratory practices and concentration ranges.

Q2: What if my stock concentration is in a different unit?
A: Convert all concentrations to consistent units before calculation. Ensure stock concentration is in mM and final concentration is in μM.

Q3: Can this calculator handle very small volumes?
A: Yes, the calculator can handle microliter volumes, but practical limitations of pipetting accuracy should be considered for very small volumes.

Q4: What's the maximum precision of the calculation?
A: The calculator provides results with 4 decimal places, but actual measurement precision depends on your laboratory equipment.

Q5: Is this formula specific to Tocris products?
A: While named after Tocris, this is a general dilution formula that can be applied to any stock solution preparation.