1. What is the Dilution Equation?

The dilution equation calculates the final concentration of a solution after dilution. It is based on the principle of conservation of mass, where the amount of solute remains constant before and after dilution.

2. How Does the Calculator Work?

The calculator uses the dilution equation:

Where:

• \( C_f \) — Final concentration (μg/mL)
• \( C_i \) — Initial concentration (μg/mL)
• \( V_i \) — Initial volume (mL)
• \( V_f \) — Final volume (mL)

Explanation: The equation calculates the concentration after dilution by multiplying the initial concentration by the dilution factor (initial volume divided by final volume).

3. Importance of Dilution Calculation

Details: Accurate dilution calculations are essential in laboratory settings, pharmaceutical preparations, and chemical experiments to achieve desired concentrations for various applications.

4. Using the Calculator

Tips: Enter initial concentration in μg/mL, initial volume in mL, and final volume in mL. All values must be valid (greater than 0).

5. Frequently Asked Questions (FAQ)

Q1: What units should I use for the inputs?
A: Use μg/mL for concentration and mL for volumes. Ensure all units are consistent for accurate results.

Q2: Can I use this calculator for other concentration units?
A: While the calculator is designed for μg/mL, you can use any concentration unit as long as all inputs use the same unit.

Q3: What if my final volume is less than initial volume?
A: The equation still works mathematically, but physically this would represent concentration rather than dilution (since V_f < V_i implies evaporation or removal of solvent).

Q4: How precise are the calculations?
A: The calculator provides results with 4 decimal places for precision, but actual precision depends on your measurement accuracy.

Q5: Can this be used for serial dilutions?
A: This calculator calculates single-step dilutions. For serial dilutions, you would need to perform multiple calculations sequentially.