1. What is the Dilution Formula?

The dilution formula calculates the final concentration of a solution after dilution. It is based on the principle of conservation of mass, where the amount of solute remains constant before and after dilution.

2. How Does the Calculator Work?

The calculator uses the dilution formula:

Where:

• \( C_f \) — Final concentration (μg/μL)
• \( C_i \) — Initial concentration (μg/μL)
• \( V_i \) — Initial volume (μL)
• \( V_f \) — Final volume (μL)

Explanation: The formula calculates the new concentration after diluting an initial volume of solution to a larger final volume.

3. Importance of Dilution Calculations

Details: Accurate dilution calculations are essential in laboratory work, pharmaceutical preparations, and chemical experiments to achieve desired concentrations for various applications.

4. Using the Calculator

Tips: Enter initial concentration in μg/μL, initial volume in μL, and final volume in μL. All values must be positive numbers greater than zero.

5. Frequently Asked Questions (FAQ)

Q1: What units should I use for this calculator?
A: This calculator uses μg/μL for concentration and μL for volume. Make sure all inputs are in these consistent units.

Q2: Can I use this for serial dilutions?
A: This calculator calculates single-step dilutions. For serial dilutions, you would need to perform multiple calculations sequentially.

Q3: What if my final volume is smaller than initial volume?
A: The formula still works mathematically, but practically this would represent concentration rather than dilution, which may not be physically possible without evaporation or other processes.

Q4: How precise are the results?
A: Results are rounded to 4 decimal places. The precision depends on the accuracy of your input values and measurement techniques.

Q5: Can this formula be used for other concentration units?
A: Yes, the formula works for any consistent concentration units (e.g., mg/mL, ng/μL) as long as all values use the same units.