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Total Dilution Factor Formula:
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The Total Dilution Factor (DF_t) represents the overall dilution achieved when performing multiple sequential dilution steps. It is calculated by multiplying the individual dilution factors from each step.
The calculator uses the formula:
Where:
Explanation: Each dilution factor represents the ratio of the final volume to the initial volume for that particular dilution step. The product of all individual factors gives the overall dilution.
Details: Accurate calculation of total dilution factor is crucial in laboratory settings for preparing solutions of desired concentrations, in analytical chemistry for sample preparation, and in various biological assays where precise dilutions are required.
Tips: Enter individual dilution factors separated by commas (e.g., "2, 5, 10"). All values must be positive numbers greater than zero. The calculator will multiply all factors to give the total dilution.
Q1: What is a dilution factor?
A: A dilution factor is the ratio of the final volume to the initial volume of a solution. For example, if you add 1 part sample to 4 parts diluent, the dilution factor is 5.
Q2: How do serial dilutions affect the total dilution?
A: In serial dilutions, each step's dilution factor multiplies with the previous ones, resulting in an exponential increase in the overall dilution.
Q3: Can dilution factors be less than 1?
A: No, dilution factors are always greater than or equal to 1. A factor of 1 means no dilution occurred.
Q4: How is concentration related to dilution factor?
A: The final concentration equals the initial concentration divided by the total dilution factor.
Q5: What's the difference between dilution factor and dilution ratio?
A: Dilution factor is the total volume divided by sample volume, while dilution ratio is typically expressed as sample:diluent (e.g., 1:4).