1. What is the Dilution Formula?

The dilution formula \( C_f = C_i \times \frac{V_i}{V_f} \) calculates the final concentration after dilution, where C_f is the final concentration, C_i is the initial concentration, V_i is the initial volume, and V_f is the final volume after dilution.

2. How Does the Calculator Work?

The calculator uses the dilution formula:

Where:

• \( C_f \) — Final concentration (ng/μL)
• \( C_i \) — Initial concentration (ng/μL)
• \( V_i \) — Initial volume (μL)
• \( V_f \) — Final volume (μL)

Explanation: The formula calculates how much the concentration decreases when a solution is diluted by adding more solvent.

3. Importance of Dilution Calculation

Details: Accurate dilution calculations are essential in molecular biology, chemistry, and medical laboratories for preparing samples with specific concentrations for experiments and analyses.

4. Using the Calculator

Tips: Enter initial concentration in ng/μL, initial volume in μL, and final volume in μL. All values must be positive numbers.

5. Frequently Asked Questions (FAQ)

Q1: What units should I use for this calculator?
A: This calculator uses ng/μL for concentration and μL for volume. Make sure all inputs are in consistent units.

Q2: Can I use this for other concentration units?
A: The formula works for any consistent concentration units (ng/mL, mg/L, etc.), but this calculator is specifically designed for ng/μL.

Q3: What if my final volume is smaller than initial volume?
A: The formula still applies, but this would represent concentration rather than dilution, resulting in a higher final concentration.

Q4: How accurate is this calculation?
A: The calculation is mathematically precise. Accuracy depends on the precision of your measurements and the quality of your dilution technique.

Q5: Can I calculate reverse dilutions with this?
A: Yes, you can solve for any variable by rearranging the formula, though this calculator specifically calculates final concentration.