1. What is the Sigma Aldrich Dilution Formula?

The Sigma Aldrich dilution formula calculates the volume of stock solution needed to achieve a desired concentration and volume. It's based on the principle of conservation of mass in dilution processes.

2. How Does the Calculator Work?

The calculator uses the Sigma Aldrich dilution formula:

Where:

• \( V_s \) — Stock volume (mL)
• \( C_f \) — Final concentration (M)
• \( V_f \) — Final volume (mL)
• \( C_i \) — Initial concentration (M)

Explanation: This formula calculates how much stock solution you need to dilute to achieve your desired concentration and volume.

3. Importance of Dilution Calculations

Details: Accurate dilution calculations are essential in laboratory settings for preparing solutions with precise concentrations, which is critical for experiments, assays, and various analytical procedures.

4. Using the Calculator

Tips: Enter final concentration in M, final volume in mL, and initial concentration in M. All values must be valid (greater than 0).

5. Frequently Asked Questions (FAQ)

Q1: What units should I use for this calculator?
A: The calculator uses molarity (M) for concentrations and milliliters (mL) for volumes. Make sure all your values are in these units.

Q2: Can I use this for serial dilutions?
A: This calculator calculates single dilutions. For serial dilutions, you would need to perform this calculation multiple times.

Q3: What if my stock concentration is very high?
A: The formula works regardless of concentration values, as long as C_f is less than C_i (you're diluting, not concentrating).

Q4: How precise are the results?
A: The calculator provides results with 4 decimal places, but your actual precision depends on your measuring equipment.

Q5: Can I use different concentration units?
A: The formula works with any consistent concentration units (mM, μM, etc.), but the calculator is set up for molarity (M).