1. What Is The Tocris Dilution Formula?

The Tocris dilution formula calculates the volume of stock solution needed to achieve a desired final concentration in a given total volume. This is essential for preparing accurate experimental solutions in laboratory settings.

2. How Does The Calculator Work?

The calculator uses the Tocris formula:

Where:

• \( V_{stock} \) — Volume of stock solution needed (μL)
• \( C_{final} \) — Desired final concentration (μM)
• \( V_{total} \) — Total volume of the final solution (mL)
• \( C_{stock} \) — Concentration of the stock solution (mM)

Explanation: The formula calculates the precise volume of concentrated stock solution required to achieve the target concentration in the final solution volume.

3. Importance Of Accurate Dilution

Details: Accurate dilution calculations are crucial for experimental reproducibility, ensuring correct drug concentrations in research, and maintaining consistency in biochemical assays.

4. Using The Calculator

Tips: Enter final concentration in μM, total volume in mL, and stock concentration in mM. All values must be positive numbers greater than zero.

5. Frequently Asked Questions (FAQ)

Q1: Why Convert mM To μM In The Calculation?
A: The formula requires consistent units. Since final concentration is in μM and stock is in mM, we convert mM to μM (1 mM = 1000 μM) for accurate calculation.

Q2: What If My Stock Concentration Is In Different Units?
A: Convert your stock concentration to mM before entering. For example, 1 M = 1000 mM, 1 μM = 0.001 mM.

Q3: Can This Calculator Be Used For Any Solvent?
A: Yes, the formula is universal for any solvent as long as the concentrations and volumes are accurately measured.

Q4: What Is The Typical Range For Stock Concentrations?
A: Stock concentrations typically range from 1-100 mM for most laboratory reagents and compounds.

Q5: How Precise Should My Measurements Be?
A: Use precision pipettes and calibrated equipment. For very small volumes (<10 μL), consider using serial dilutions for better accuracy.