1. What is the Stock Solution Formula?

The stock solution formula calculates the volume of concentrated stock solution needed to prepare a specific volume of solution at a desired concentration. This is essential in laboratory settings for accurate solution preparation.

2. How Does the Calculator Work?

The calculator uses the dilution formula:

Where:

• \( V_s \) — Volume of stock solution needed (mL)
• \( C_f \) — Final desired concentration (mg/mL)
• \( V_f \) — Final volume of solution (mL)
• \( C_s \) — Concentration of stock solution (mg/mL)

Explanation: This formula is derived from the principle of mass conservation, where the amount of solute remains constant before and after dilution.

3. Importance of Stock Solution Preparation

Details: Accurate solution preparation is crucial for experimental consistency, reproducibility of results, and ensuring proper concentrations for biological assays, chemical reactions, and medical applications.

4. Using the Calculator

Tips: Enter all concentrations in mg/mL and volumes in mL. Ensure all values are positive numbers. The calculator will determine the volume of stock solution needed to achieve your desired final concentration and volume.

5. Frequently Asked Questions (FAQ)

Q1: Can I use different concentration units?
A: The calculator uses mg/mL consistently. Convert all values to mg/mL before calculation for accurate results.

Q2: What if my stock concentration is very high?
A: The formula works for any concentration values as long as units are consistent. Very high stock concentrations will result in very small required volumes.

Q3: How precise should my measurements be?
A: Precision depends on your application. For critical experiments, use analytical balances and precision glassware for the most accurate results.

Q4: Can this formula be used for serial dilutions?
A: Yes, this formula is the foundation for preparing serial dilutions, though each dilution step requires separate calculation.

Q5: What if I need to prepare a solution from solid solute?
A: For preparing solutions from solid compounds, use: mass = concentration × volume. This calculator is specifically for diluting existing stock solutions.