Tocris Stock Dilution Calculator

Tocris Dilution Formula:

\[ V_s = \frac{C_f \times V_f}{C_s} \]

Stock Volume (V_s):

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1. What is the Tocris Stock Dilution Formula?

The Tocris stock dilution formula calculates the volume of stock solution needed to achieve a desired final concentration in a given volume. This is essential for preparing working solutions from concentrated stock solutions in laboratory settings.

2. How Does the Calculator Work?

The calculator uses the Tocris dilution formula:

\[ V_s = \frac{C_f \times V_f}{C_s} \]

Where:

\( V_s \) — Stock volume (μL)
\( C_f \) — Final concentration (μM)
\( V_f \) — Final volume (mL)
\( C_s \) — Stock concentration (mM)

Explanation: The formula calculates the required volume of concentrated stock solution to dilute to achieve the desired final concentration in the specified volume.

3. Importance of Stock Dilution Calculation

Details: Accurate dilution calculations are crucial for preparing precise concentrations of reagents, drugs, or compounds in experimental work, ensuring reproducibility and reliability of results.

4. Using the Calculator

Tips: Enter final concentration in μM, final volume in mL, and stock concentration in mM. All values must be positive numbers greater than zero.

5. Frequently Asked Questions (FAQ)

Q1: Why use this specific formula for stock dilution?
A: This formula is specifically designed for calculating stock volumes when working with Tocris compounds and follows standard dilution principles used in laboratory settings.

Q2: What units should I use for the inputs?
A: Use μM for final concentration, mL for final volume, and mM for stock concentration. The calculator will output stock volume in μL.

Q3: Can I use this calculator for other brands besides Tocris?
A: Yes, the dilution formula is universal and can be used for any stock solution dilution calculation, regardless of the brand.

Q4: What if my stock concentration is in a different unit?
A: Convert your stock concentration to mM before entering the value. For example, 1 M = 1000 mM, 1 μM = 0.001 mM.

Q5: How precise are the calculations?
A: The calculator provides results with 2 decimal places precision, but actual laboratory precision depends on your measurement equipment and techniques.